Trevor Lewis

Senior Lecturer

Dr. Trevor Lewis is a senior lecturer in Physiology within the School of Medical Sciences. He teaches across the science, medical science and medicine programs in physiology and neuroscience. His research explores the relationship between structure and function in ligand-gated ion channels and more broadly the processes underlying cellular excitability. This is investigated with the application of patch clamp electrophysiology to record whole-cell and single channel responses, and imaging techniques for localisation of ion channels. Previous research has contributed to understanding of the molecular events leading to the opening of ligand-gated ion channels (channel gating), through the investigation of disease mutations in human glycine receptors, and the understanding ligand interactions at neuronal nicotinic acetylcholine receptors. The current focus is on novel mechanisms for activating ligand-gated ion channels at interfaces between channel subunits not typically involved in ligand binding.

Journal articles
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Indurthi DC; Qudah T; Liao VW; Ahring PK; Lewis TM; Balle T; Chebib M; Absalom NL, 2019, 'Revisiting autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) mutations in the nicotinic acetylcholine receptor reveal an increase in efficacy regardless of stochiometry', Pharmacological Research, vol. 139, pp. 215 - 227, http://dx.doi.org/10.1016/j.phrs.2018.11.031
2019
Polly P; Thai T; Yang JL; Luo A; Herbert C; Jones N; Lewis T; Vickery R; Richardson A; Schibeci S, 2018, 'The teacher-student journey: Program-wide teamwork skills development and evaluation in the medical sciences', International Journal of Assessment and Evaluation, vol. 24, pp. 1 - 24, http://dx.doi.org/10.18848/2327-7920/CGP/v24i04/1-24
2018
Duncan T; Lowe A; Sidhu K; Sachdev P; Lewis T; Lin RCY; Sytnyk V; Valenzuela M, 2017, 'Replicable Expansion and Differentiation of Neural Precursors from Adult Canine Skin.', Stem Cell Reports, vol. 9, pp. 557 - 570, http://dx.doi.org/10.1016/j.stemcr.2017.07.008
2017
Lewis TM; Indurthi DC; Ahring PK; Balle T; Chebib M; Absalom NL, 2016, 'Ligand Binding at the α4-α4 Agonist-Binding Site of the α4β2 nAChR Triggers Receptor Activation through a Pre-Activated Conformational State', PLoS One, vol. 11, pp. e0161154 - e0161154, http://dx.doi.org/10.1371/journal.pone.0161154
2016
Carland JE; Yamamoto I; Hanrahan JR; Abdel-Halim H; Lewis TM; Absalom N; Chebib M, 2014, 'A Hydrophobic Area of the GABA ρ1 Receptor Containing Phenylalanine 124 Influences Both Receptor Activation and Deactivation', Journal of Molecular Neuroscience, vol. 55, pp. 305 - 313, http://dx.doi.org/10.1007/s12031-014-0322-7
2014
Barry PH; Lewis TM; Moorhouse AJ, 2013, 'An optimised 3 M KCl salt-bridge technique used to measure and validate theoretical liquid junction potential values in patch-clamping and electrophysiology', European Biophysics Journal, vol. 42, pp. 631 - 646, http://dx.doi.org/10.1007/s00249-013-0911-3
2013
Absalom NL; Quek G; Lewis TM; Qudah T; Von Arenstorff I; Ambrus JI; Harpsøe K; Karim N; Balle T; McLeod MD; Chebib M, 2013, 'Covalent trapping of methyllycaconitine at the α4-α4 interface of the α4α2 nicotinic acetylcholine receptor: Antagonist binding site and mode of receptor inhibition revealed', Journal of Biological Chemistry, vol. 288, pp. 26521 - 26532, http://dx.doi.org/10.1074/jbc.M113.475053
2013
Huang SH; Lewis TM; Lummis SCR; Thompson AJ; Chebib M; Johnston GAR; Duke RK, 2012, 'Mixed antagonistic effects of the ginkgolides at recombinant human ρ1 GABAC receptors', Neuropharmacology, vol. 63, pp. 1127 - 1139, http://dx.doi.org/10.1016/j.neuropharm.2012.06.067
2012
Cederholm JME; Absalom NL; Sugiharto S; Griffith R; Schofield PR; Lewis TM, 2010, 'Conformational changes in extracellular loop 2 associated with signal transduction in the glycine receptor', Journal of Neurochemistry, vol. 115, pp. 1245 - 1255, http://dx.doi.org/10.1111/j.1471-4159.2010.07021.x
2010
Sugiharto S; Carland JE; Lewis TM; Moorhouse AJ; Barry PH, 2010, 'External divalent cations increase anion-cation permeability ratio in glycine receptor channels', Pflugers Archiv - European Journal of Physiology, vol. 460, pp. 131 - 152, http://dx.doi.org/10.1007/s00424-010-0792-6
2010
Barry PH; Sugiharto S; Lewis TM; Moorhouse AJ, 2010, 'Further analysis of counterion permeation through anion-selective glycine receptor channels.', Channels, vol. 4, pp. 142 - 149, http://www.landesbioscience.com/journals/channels/article/11020
2010
Carland JE; cooper MA; Sugiharto S; Jeong H; Lewis TM; Barry PH; Peters JA; Lambert J; Moorhouse AJ, 2009, 'Characterization of the effects of charged residues in the intracellular loop on ion permeation in α1 glycine receptor channels', The Journal of Biological Chemistry, vol. 284, pp. 2023 - 2030, http://dx.doi.org/10.1074/jbc.M806618200
2009
Cederholm JME; Schofield PR; Lewis TM, 2009, 'Gating mechanisms in Cys-loop receptors', European Biophysics Journal, vol. 39, pp. 37 - 49, http://dx.doi.org/10.1007/s00249-009-0452-y
2009
Absalom NL; Schofield PR; Lewis TM, 2009, 'Pore structure of the Cys-loop ligand-gated ion channels', Neurochemical Research, vol. 34, pp. 1805 - 1815, http://dx.doi.org/10.1007/s11064-009-9971-2
2009
Sugiharto S; Lewis TM; Moorhouse AJ; Schofield PR; Barry PH, 2008, 'Anion-cation permeability correlates with hydrated counterion size in glycine receptor channels', Biophysical Journal, vol. 95, pp. 4698 - 4715, http://dx.doi.org/10.1529/biophysj.107.125690
2008
Qu W; Moorhouse AJ; Chandra M; Pierce KD; Lewis TM; Barry PH, 2006, 'A single P-loop glutamate point mutation to either lysine or arginine switches the cation-anion selectivity of the CNGA2 channel', Journal of General Physiology, vol. 127, pp. 375 - 389, http://dx.doi.org/10.1085/jgp.200509378
2006
Qu W; Moorhouse AJ; Lewis TM; Pierce KD; Barry PH, 2005, 'Mutation of the pore glutamate affects both cytoplasmic and external dequalinium block in the rat olfactory CNGA2 channel', European Biophysics Journal with Biophysics Letters, vol. 34, pp. 442 - 453, http://dx.doi.org/10.1007/s00249-005-0479-7
2005
Morris R, 2004, 'In vivo somatic delivery of plasmid DNA and retrograde transport to obtain cell-specific gene expression in the central nervous system', Journal of Neurochemistry, vol. 90, pp. 1445 - 1452, http://dx.doi.org/10.1111/j.1471-4159.2004.02612.x
2004
Absalom NL; Lewis TM; Schofield PR, 2004, 'Mechanisms of channel gating of the ligand-gated ion channelsuperfamily inferred from protein structure', Experimental Physiology, vol. 89, pp. 145 - 153, http://dx.doi.org/10.1113/expphysiol.2003.026815
2004
Absalom NL; Lewis TM; Kaplan W; Pierce KD; Schofield PR, 2003, 'Role of charged residues in coupling ligand binding and channel activation in the extracellular domain of the clycine receptor', The Journal of Biological Chemistry, vol. 278, pp. 50151 - 50157, http://dx.doi.org/10.1074/jbc.M305357200
2003
Lewis TM; Schofield PR; McClellan AM, 2003, 'Kinetic determinants of agonist potency at the recombinant human glycine receptor', The Journal of Physiology, vol. 549, pp. 361 - 374, http://dx.doi.org/10.1113/jphysiol.2002.037796
2003
Rees MI; Lewis TM; Kwok JB; Mortier GR; Govaert P; Snell RG; Schofield PR; Owen MJ, 2002, 'Hyperekplexia associated with compound heterozygote mutations in the β-subunit of the human inhibitory glycine receptor (GLRB).', Human Molecular Genetics, vol. 11, pp. 853 - 860, http://dx.doi.org/10.1093/hmg/11.7.853
2002
Rees MI; Lewis TM; Vafa B; Ferrie C; Corry P; Muntoni F; Jungbluth H; Stephenson JB; Kerr M; Snell RG; Schofield PR; Owen MJ, 2001, 'Compound heterozygosity and nonsense mutations in the α1-subunit of the inhibitory glycine receptor in hyperekplexia', Human Genetics, vol. 109, pp. 267 - 270, http://dx.doi.org/10.1007/s004390100569
2001
Rees MI; Lewis TM; Mortier G; Snell RG; Schofield PR; Owen MJ, 2000, 'A transient hyperekplexia phenotype associated with compound heterozygote mutations in the human beta-subunit of the inhibitory glycine receptor (GLRB).', AMERICAN JOURNAL OF HUMAN GENETICS, vol. 67, pp. 391 - 391, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000089400702194&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
2000
Vafa B; Lewis TM; Cunningham AM; Jacques P; Lynch JP; Schofield PR, 1999, 'Identification of a new ligand binding domain in the alpha1 subunit of the inhibitory glycine receptor', Journal of Neurochemistry, vol. 73, pp. 2158 - 2166
1999
Lewis TM; Schofield PR, 1999, 'Structure-function relationships of the human glycine receptor: Insights from hyperekplexia mutations', Annals of the New York Academy of Sciences, vol. 868, pp. 681 - 684, http://dx.doi.org/10.1111/j.1749-6632.1999.tb11345.x
1999
Lewis TM; Sivilotti LG; Colquhoun D; Gardiner RM; Schoepfer R; Rees M, 1998, 'Properties of human glycine receptors containing the hyperekplexia mutation α1 (K276E), expressed in Xenopus oocytes', Journal of Physiology, vol. 507, pp. 25 - 40, http://dx.doi.org/10.1111/j.1469-7793.1998.025bu.x
1998
Sivilotti LG; McNeil DK; Lewis TM; Nassar MA; Schoepfer R; Colquhoun D, 1997, 'Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour', Journal of Physiology, vol. 500, pp. 123 - 138, http://dx.doi.org/10.1113/jphysiol.1997.sp022004
1997
Lewis TM; Harkness PC; Sivilotti LG; Colquhoun D; Millar NS, 1997, 'The ion channel properties of a rat recombinant neuronal nicotinic receptor are dependent on the host cell type', Journal of Physiology, vol. 505, pp. 299 - 306, http://dx.doi.org/10.1111/j.1469-7793.1997.299bb.x
1997
Lewis TM; Roberts ML; Bretag AH, 1994, 'Immunolabelling for VDAC, the mitochondrial voltage-dependent anion channel, on sarcoplasmic reticulum from amphibian skeletal muscle', Neuroscience Letters, vol. 181, pp. 83 - 86, http://dx.doi.org/10.1016/0304-3940(94)90565-7
1994
Lewis TM; Dulhunty AF; Junankar PR; Stanhope C, 1992, 'Ultrastructure of sarcoballs on the surface of skinned amphibian skeletal muscle fibres', Journal of Muscle Research and Cell Motility, vol. 13, pp. 640 - 653, http://dx.doi.org/10.1007/BF01738254
1992
Book Chapters
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Polly P; Vickery R; Thai T; Yang JL; Fath T; Herbert C; Jones N; Lewis T; Pather N; Schibeci S; Cox J, 2016, 'ePortfolios, Assessment and Professional Skills in the Medical Sciences', in Rowley J (ed.), ePortfolios in Australian Universities, Springer, Singapore, pp. 47 - 64, http://dx.doi.org/10.1007/978-981-10-1732-2_4
2016
Moorhouse AJ; Lewis TM; Barry PH, 2015, 'Analyzing Ion Permeation in Channels and Pumps Using Patch-Clamp Recording', in Clarke RJ; Khalid MAA (ed.), Pumps, Channels and Transporters Methods of Functional Analysis, John Wiley & Sons, Hoboken, New Jersey, pp. 51 - 88, http://dx.doi.org/10.1002/9781119085126.ch3
2015
Conference Abstracts
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Indurthil DC; Salle T; Ahringl PK; Chebibl M; Lewis TM; Absalom NL, 2015, 'Exploiting ligand selectivity to understand allosteric receptor opening', in BIOCHEMICAL PHARMACOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, Chicago, IL, Vol. 97, pp. 624 - 624, presented at Conference on Nicotinic Acetylcholine Receptors as Therapeutic Targets - Emerging Frontiers in Basic Research and Clinical Science, Chicago, IL, 14 October 2015 - 15 October 2015, http://dx.doi.org/10.1016/j.bcp.2015.08.012
2015
Cederholm J; Absalom N; Sugiharto S; Griffith R; Schofield P; Lewis T, 2009, 'Loop 2 in the extracellular domain of the glycine receptor is involved in signal transduction', in Proceedings of Society for Neuroscience, Chicago, IL, USA., presented at Society for Neuroscience 2009, Chicago, IL, USA.
2009
Conference Posters
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Windley MJ; Drwal M; Lewis TM, 2014, 'A novel startle disease mutation reveals the significance of W170 in glycine receptor function', Mt Holyoke College, MA, USA, presented at Gordon Research Conference. Ion Channels: The Molecular Basis of Excitability and Disease., Mt Holyoke College, MA, USA, 06 July 2014 - 11 July 2014
2014
Conference Presentations
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Barry PH; Lewis TM; Moorhouse AJ, 2011, 'An improved method, with theoretical analysis, for the simple experimental measurement of liquid junction potentials', presented at Joint ASB-BioPhysChem 2011 Conference, University of Wollongong, NSW, Australia, 03 December 2011 - 06 December 2011
2011
Cederholm J; Absalom N; Sugiharto S; Griffith R; Schofield P; Lewis T, 2011, 'Conformational changes in extracellular loop 2 associated with signal transduction in the glycine receptor', presented at 23rd ISN-ESN Biennial Meeting, Athens, Greece, 28 August 2011 - 01 September 2011, https://www.neurochemistry.org/wp-content/uploads/2018/10/2011-Programme-book.pdf
2011

2019 Faculty of Medicine Educator of the Year

2019 Faculty of Medicine Award for Teaching Excellence

2018 School of Medical Science Teaching Award - Outstanding contributions t the student experience

2015 UNSW Vice Chancellor's Award for Teaching Excellence - Outstanding contributions to student learning

Structure and Function of Ion Channels, Ligand Gated Ion Channels, Patch Clamp Electrophysiology, Homology Modelling

MFAC1522 Beginnings, Growth and Development B, co-convenor
NEUR3121 Molecular and Cellular Neuroscience, convenor
PHAR3102 Molecular Pharmacology
Endocrine physiology components of MFAC1521/MFAC1522 Beginnings, Growth and Development, PHSL2201/PHSL2221/PHSL2502 Physiology 1B