David Jacques

Scientia Fellow (Level C)

David leads the Structural Virology Group at the EMBL Australia Node for Single Molecule Science. Having completed a bachelor of science majoring in biochemistry and chemistry, David undertook his PhD under the supervision of Professors Jill Trewhella and Mitchell Guss at the University of Sydney. During that time, David employed both crystallography and small-angle scattering to understand protein structure. He also authored the internationally accepted standards by which biological small-angle scattering data are now reported in publications. In 2012, the award of an NHMRC Early Career Fellowship enabled him to join the group of Leo James at the MRC Laboratory of Molecular Biology, Cambridge. Here he studied the role played by the HIV capsid during the early stage of infection, revealing structural dynamics and interactions with the host at a molecular level not previously seen. Since returning to Australia, David leads an independent research team focused on understanding the molecular details of host-pathogen interactions through the use of crystallographic, single molecule fluorescence, and cryo-electron microscopy methods.

Journal articles
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Lau D; Walsh JC; Peng W; Shah VB; Turville S; Jacques DA; Böcking T, 2019, 'Fluorescence Biosensor for Real-Time Interaction Dynamics of Host Proteins with HIV-1 Capsid Tubes', ACS Applied Materials and Interfaces, vol. 11, pp. 34586 - 34594, http://dx.doi.org/10.1021/acsami.9b08521
2019
James LC; Jacques DA, 2018, 'The human immunodeficiency virus capsid is more than just a genome package', Annual Review of Virology, vol. 5, pp. 209 - 225, http://dx.doi.org/10.1146/annurev-virology-092917-043430
2018
Mallery DL; Márquez CL; McEwan WA; Dickson CF; Jacques DA; Anandapadamanaban M; Bichel K; Towers GJ; Saiardi A; Böcking T; James LC, 2018, 'IP6 is an HIV pocket factor that prevents capsid collapse and promotes DNA synthesis', eLife, vol. 7, http://dx.doi.org/10.7554/eLife.35335
2018
Márquez CL; Lau D; Walsh J; Shah V; McGuinness C; Wong A; Aggarwal A; Parker MW; Jacques DA; Turville S; Böcking T, 2018, 'Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis', eLife, vol. 7, http://dx.doi.org/10.7554/eLife.34772
2018
Bhati M; Llamosas E; Jacques DA; Jeffries CM; Dastmalchi S; Ripin N; Nicholas HR; Matthews JM, 2017, 'Interactions between LHX3-And ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans', Scientific Reports, vol. 7, http://dx.doi.org/10.1038/s41598-017-04587-8
2017
Trewhella J; Duff AP; Durand D; Gabel F; Guss JM; Hendrickson WA; Hura GL; Jacques DA; Kirby NM; Kwan AH; Pérez J; Pollack L; Ryan TM; Sali A; Schneidman-Duhovny D; Schwede T; Svergun DI; Sugiyama M; Tainer JA; Vachette P; Westbrook J; Whitten AE, 2017, '2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: An update', Acta Crystallographica Section D: Structural Biology, vol. 73, pp. 710 - 728, http://dx.doi.org/10.1107/S2059798317011597
2017
Jacques DA; McEwan WA; Hilditch L; Price AJ; Towers GJ; James LC, 2016, 'HIV-1 uses dynamic capsid pores to import nucleotides and fuel encapsidated DNA synthesis', Nature, vol. 536, pp. 349 - 353, http://dx.doi.org/10.1038/nature19098
2016
Dickson CF; Jacques DA; Clubb RT; Guss JM; Gell DA, 2015, 'The structure of haemoglobin bound to the haemoglobin receptor IsdH from Staphylococcus aureus shows disruption of the native α-globin haem pocket', Acta Crystallographica Section D: Biological Crystallography, vol. 71, pp. 1295 - 1306, http://dx.doi.org/10.1107/S1399004715005817
2015
Wood K; Jeffries CM; Knott RB; Sokolova A; Jacques DA; Duff AP, 2015, 'Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO', Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, vol. 798, pp. 44 - 51, http://dx.doi.org/10.1016/j.nima.2015.06.034
2015
Wilkinson-White L; Lester KL; Ripin N; Jacques DA; Mitchell Guss J; Matthews JM, 2015, 'GATA1 directly mediates interactions with closely spaced pseudopalindromic but not distantly spaced double GATA sites on DNA', Protein Science, vol. 24, pp. 1649 - 1659, http://dx.doi.org/10.1002/pro.2760
2015
Tam JCH; Jacques DA, 2014, 'Intracellular immunity: Finding the enemy within-how cells recognize and respond to intracellular pathogens', Journal of Leukocyte Biology, vol. 96, pp. 233 - 244, http://dx.doi.org/10.1189/jlb.4RI0214-090R
2014
Dickson CF; Kumar KK; Jacques DA; Malmirchegini GR; Spirig T; Mackay JP; Clubb RT; Guss JM; Gell DA, 2014, 'Structure of the hemoglobin-isdh complex reveals the molecular basis of iron capture by staphylococcus aureus', Journal of Biological Chemistry, vol. 289, pp. 6728 - 6738, http://dx.doi.org/10.1074/jbc.M113.545566
2014
Price AJ; Jacques DA; McEwan WA; Fletcher AJ; Essig S; Chin JW; Halambage UD; Aiken C; James LC, 2014, 'Host Cofactors and Pharmacologic Ligands Share an Essential Interface in HIV-1 Capsid That Is Lost upon Disassembly', PLoS Pathogens, vol. 10, http://dx.doi.org/10.1371/journal.ppat.1004459
2014
Kumar KK; Jacques DA; Guss JM; Gell DA, 2014, 'The structure of α-haemoglobin in complex with a haemoglobin-binding domain from Staphylococcus aureus reveals the elusive α-haemoglobin dimerization interface', Acta Crystallographica Section F:Structural Biology Communications, vol. 70, pp. 1032 - 1037, http://dx.doi.org/10.1107/S2053230X14012175
2014
Vandevenne M; Jacques DA; Artuz C; Nguyen CD; Kwan AH; Segal DJ; Matthews JM; Crossley M; Guss JM; Mackay JP, 2013, 'New insights into DNA recognition by zinc fingers revealed by structural analysis of the oncoprotein ZNF217', J Biol Chem, vol. 288, pp. 10616 - 10627, http://dx.doi.org/10.1074/jbc.M112.441451
2013
Gadd MS; Jacques DA; Nisevic I; Craig VJ; Kwan AH; Guss JM; Matthews JM, 2013, 'A structural basis for the regulation of the LIM-homeodomain protein islet 1 (Isl1) by intra- and intermolecular interactions', Journal of Biological Chemistry, vol. 288, pp. 21924 - 21935, http://dx.doi.org/10.1074/jbc.M113.478586
2013
Rasaiyaah J; Tan CP; Fletcher AJ; Price AJ; Blondeau C; Hilditch L; Jacques DA; Selwood DL; James LC; Noursadeghi M; Towers GJ, 2013, 'HIV-1 evades innate immune recognition through specific cofactor recruitment', Nature, vol. 503, pp. 402 - 405, http://dx.doi.org/10.1038/nature12769
2013
Jacques DA; Guss JM; Svergun DI; Trewhella J, 2012, 'Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution', Acta Crystallographica Section D: Biological Crystallography, vol. 68, pp. 620 - 626, http://dx.doi.org/10.1107/S0907444912012073
2012
Gadd MS; Jacques DA; Guss JM; Matthews JM, 2012, 'Crystallization and diffraction of an Isl1-Ldb1 complex', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 68, pp. 1398 - 1401, http://dx.doi.org/10.1107/S1744309112040031
2012
Jacques DA; Guss JM; Trewhella J, 2012, 'Reliable structural interpretation of small-angle scattering data from bio-molecules in solution - The importance of quality control and a standard reporting framework', BMC Structural Biology, vol. 12, http://dx.doi.org/10.1186/1472-6807-12-9
2012
Kumar KK; Jacques DA; Pishchany G; Caradoc-Davies T; Spirig T; Malmirchegini GR; Langley DB; Dickson CF; Mackay JP; Clubb RT; Skaar EP; Guss JM; Gell DA, 2011, 'Structural basis for hemoglobin capture by Staphylococcus aureus cell-surface protein, IsdH', Journal of Biological Chemistry, vol. 286, pp. 38439 - 38447, http://dx.doi.org/10.1074/jbc.M111.287300
2011
Jacques DA; Langley DB; Kuramitsu S; Yokoyama S; Trewhella J; Guss JM, 2011, 'The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolase', Acta Crystallographica Section D: Biological Crystallography, vol. 67, pp. 105 - 111, http://dx.doi.org/10.1107/S0907444910051127
2011
Jacques DA; Langley DB; Hynson RMG; Whitten AE; Kwan A; Guss JM; Trewhella J, 2011, 'A novel structure of an antikinase and its inhibitor', Journal of Molecular Biology, vol. 405, pp. 214 - 226, http://dx.doi.org/10.1016/j.jmb.2010.10.047
2011
Jacques DA; Trewhella J, 2010, 'Small-angle scattering for structural biology - Expanding the frontier while avoiding the pitfalls', Protein Science, vol. 19, pp. 642 - 657, http://dx.doi.org/10.1002/pro.351
2010
Hynson RMG; Kwan AH; Jacques DA; MacKay JP; Trewhella J, 2010, '1H, 13C and 15N backbone and side chain resonance assignments of the N-terminal domain of the histidine kinase inhibitor KipI from Bacillus subtilis', Biomolecular NMR Assignments, vol. 4, pp. 167 - 169, http://dx.doi.org/10.1007/s12104-010-9237-6
2010
Jacques DA; Streamer M; Rowland SL; King GF; Guss JM; Trewhella J; Langley DB, 2009, 'Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state', Acta Crystallographica Section D: Biological Crystallography, vol. 65, pp. 574 - 581, http://dx.doi.org/10.1107/S090744490901169X
2009
Jacques DA; Langley DB; Jeffries CM; Cunningham KA; Burkholder WF; Guss JM; Trewhella J, 2008, 'Histidine Kinase Regulation by a Cyclophilin-like Inhibitor', Journal of Molecular Biology, vol. 384, pp. 422 - 435, http://dx.doi.org/10.1016/j.jmb.2008.09.017
2008
Whitten AE; Jacques DA; Hammouda B; Hanley T; King GF; Guss JM; Trewhella J; Langley DB, 2007, 'The Structure of the KinA-Sda Complex Suggests an Allosteric Mechanism of Histidine Kinase Inhibition', Journal of Molecular Biology, vol. 368, pp. 407 - 420, http://dx.doi.org/10.1016/j.jmb.2007.01.064
2007
Finnie KS; Jacques DA; McGann MJ; Blackford MG; Barbé CJ, 2006, 'Encapsulation and controlled release of biomolecules from silica microparticles', Journal of Materials Chemistry, vol. 16, pp. 4494 - 4498, http://dx.doi.org/10.1039/b611840b
2006

2018-2020: ARC Discovery Project DP180101384 "Regulation of DNA Synthesis and Host Evasion by Lentivirus Capsids"